goat anti mouse 750 group 4 cd4 Search Results


goat anti mouse 750 group 4 cd4  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc goat anti mouse 750 group 4 cd4
Goat Anti Mouse 750 Group 4 Cd4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse 750 group 4 cd4/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
goat anti mouse 750 group 4 cd4 - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
goat anti-rabbit alexa594 secondary  (Thermo Fisher)
90
Thermo Fisher goat anti-rabbit alexa594 secondary
Goat Anti Rabbit Alexa594 Secondary, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti-rabbit alexa594 secondary/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
goat anti-rabbit alexa594 secondary - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
rabbit anti-iba1  (FUJIFILM)
90
FUJIFILM rabbit anti-iba1
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Rabbit Anti Iba1, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-iba1/product/FUJIFILM
Average 90 stars, based on 1 article reviews
rabbit anti-iba1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
anti-influenza neutralizing antibody (goat anti-influenza a/ussr (h1n1  (Millipore)
90
Millipore anti-influenza neutralizing antibody (goat anti-influenza a/ussr (h1n1
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Anti Influenza Neutralizing Antibody (Goat Anti Influenza A/Ussr (H1n1, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-influenza neutralizing antibody (goat anti-influenza a/ussr (h1n1/product/Millipore
Average 90 stars, based on 1 article reviews
anti-influenza neutralizing antibody (goat anti-influenza a/ussr (h1n1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
goat anti rat igg 555 cd8  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc goat anti rat igg 555 cd8
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Goat Anti Rat Igg 555 Cd8, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti rat igg 555 cd8/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
goat anti rat igg 555 cd8 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
goat anti rabbit igg 647 cd56  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc goat anti rabbit igg 647 cd56
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Goat Anti Rabbit Igg 647 Cd56, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti rabbit igg 647 cd56/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
goat anti rabbit igg 647 cd56 - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
goat anti rabbit igg hrp tsa 555 cd11c  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc goat anti rabbit igg hrp tsa 555 cd11c
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Goat Anti Rabbit Igg Hrp Tsa 555 Cd11c, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti rabbit igg hrp tsa 555 cd11c/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
goat anti rabbit igg hrp tsa 555 cd11c - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
goat anti rabbit igg hrp tsa 488 cd20  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc goat anti rabbit igg hrp tsa 488 cd20
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Goat Anti Rabbit Igg Hrp Tsa 488 Cd20, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti rabbit igg hrp tsa 488 cd20/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
goat anti rabbit igg hrp tsa 488 cd20 - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
anti pig albumin antibody  (Bethyl)
93
Bethyl anti pig albumin antibody
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Anti Pig Albumin Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pig albumin antibody/product/Bethyl
Average 93 stars, based on 1 article reviews
anti pig albumin antibody - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
sapnf  (Millipore)
90
Millipore sapnf
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Sapnf, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sapnf/product/Millipore
Average 90 stars, based on 1 article reviews
sapnf - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
ampk cell signaling technology  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc ampk cell signaling technology
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Ampk Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ampk cell signaling technology/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
ampk cell signaling technology - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
pi3k cell signaling technology  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc pi3k cell signaling technology
(A) Left panels: Representative images of tissue sections probed for microglia <t>(Iba1:</t> TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall <t>Iba1</t> <t>positive</t> objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.
Pi3k Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pi3k cell signaling technology/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
pi3k cell signaling technology - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier

Image Search Results


(A) Left panels: Representative images of tissue sections probed for microglia (Iba1: TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall Iba1 positive objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.

Journal: PLOS ONE

Article Title: Early chronic suppression of microglial p38α in a model of Alzheimer’s disease does not significantly alter amyloid-associated neuropathology

doi: 10.1371/journal.pone.0286495

Figure Lengend Snippet: (A) Left panels: Representative images of tissue sections probed for microglia (Iba1: TxRed [red]), fibrillar amyloid deposits (Thioflavin S: FITC [green]), and Aβ peptides (6E10: Cy5 [purple]) obtained on a Nikon confocal microscope. Z-stack images across 18 μm were obtained for each plaque of interest and post-processed using NIS-Elements software (n = 10 animals per group, 20 plaques per animal). Right panels: Images were imported into Imaris software, where individual surface objects were detected using built-in algorithms manually thresholded for signal intensity and object size. For analysis of plaque-associated microglia, a region of interest restricting measures to only those cells residing within 15 μm of the plaque was applied to each image (white semi-translucent region). Right inset: Close up of an Imaris reconstruction showing a plaque-associated microglial cell. Aβ peptides (purple objects) contained within the cell body are indicated by yellow arrows. (B) Prior to analysis of plaque-associated microglia, overall Iba1 positive objects in the hippocampus were obtained for each section (n = 8–10 per group). No change was detected between groups (Student’s t -Test; p ≥ 0.05). (C, D) The number of microglia residing within 15 μm of the plaques did not change in response to p38α suppression (Student’s t -Test; p ≥ 0.05). However, quantification of Aβ volume (μm 3 ) in plaque-associated microglia revealed a significant decrease in AD p38 KO mice compared to p38 +/+ animals (Student’s t -Test; p = 0.011), suggesting that p38α suppression may potentially affect microglia-plaque dynamics and/or phagocytic processes in this region. Data represent means ± SEM. * p ≤ 0.05.

Article Snippet: For immunofluorescent analyses, tissue sections from AD mice (n = 8–10 mice per group, 4–10 sections per animal) were selected and blocked as above, then incubated overnight in 1:2000 rabbit anti-Iba1 (FUJIFILM Wako Shibayagi, #019–19741, RRID:AB_839504), 1:800 Alexa647-conjugated mouse anti-Aβ 6E10 (BioLegend, #803021, RRID:AB_2783374), and 1:1000 goat anti-rabbit Alexa594 secondary (Thermo Fisher Scientific, #A-11012, RRID:AB_2534079).

Techniques: Microscopy, Software